| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071514 |
| Chromosome: | chromosome 6 |
| Location: | 5505912 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGTAATACATTTCATGCATAGCCGCTTGCAATACCGCTGGCCTAACTC |
| Internal bar code: | TTTTTAATAGGGAGGAACGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2594 |
| LEAP-Seq percent confirming: | 98.9691 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGCAGGAAGTGAGGAGG |
| Suggested primer 2: | CTGACGGAGGAGGAGATTGC |