| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071516 |
| Chromosome: | chromosome 12 |
| Location: | 3218072 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499850 | CNK7 | NimA-related protein kinase; (1 of 15) K08857 - NIMA (never in mitosis gene a)-related kinase [EC:2.7.11.1] (NEK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGACACAGGCTGTTTCAGAGCCGCTCAGCCTCTGGAGGGATCTGCAC |
| Internal bar code: | GATGATGCCGGTGGGAATGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4438 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTAGTGGTGAATGCTGGT |
| Suggested primer 2: | CGCTGTTAGTGTGGGGACAT |