| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071526 |
| Chromosome: | chromosome 9 |
| Location: | 2539379 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392800 | POB24 | Proteome of basal body 24 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACCGCGCCTTGTGCCTTCGGGTGGGGGGCTCTGACAGCTCGGACAGG |
| Internal bar code: | AAATTGTGGATTAGCACGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2606 |
| LEAP-Seq percent confirming: | 96.7213 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAATGGCCAGTTGACCTC |
| Suggested primer 2: | ATGGATCTCACGATGGACGC |