| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071529 |
| Chromosome: | chromosome 3 |
| Location: | 1970187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155550 | DNJ18 | (1 of 1) IPR001623//IPR012336 - DnaJ domain // Thioredoxin-like fold; DnaJ-like protein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCGGTTCGTGGCGGTCAACACAAGGCGAGTCAACGCTGGCCCGCCTT |
| Internal bar code: | CGTGTATTCAAGCGTCGTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4874 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGACAACGTCTTGGGTG |
| Suggested primer 2: | GAGGAGTCGTTCGTGTCGTT |