| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071542 |
| Chromosome: | chromosome 7 |
| Location: | 999648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319500 | (1 of 2) 2.3.2.15 - Glutathione gamma-glutamylcysteinyltransferase / Phytochelatin synthase | 5'UTR | |
| Cre07.g319550 | (1 of 1) PF10442 - FIST C domain (FIST_C) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTTTGCTAGGCGTGTCGGTACTCCGTGCTCTGCATATGCCAAAGCGC |
| Internal bar code: | TCACAGTCCAGAAACAGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 975 |
| LEAP-Seq percent confirming: | 76.0563 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCTTGCGAGAGATGTGT |
| Suggested primer 2: | CGGGGCAGGAGAACTCAATT |