| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071551 |
| Chromosome: | chromosome 1 |
| Location: | 3208772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020575 | HSP22D | (1 of 4) PTHR11527//PTHR11527:SF128 - SMALL HEAT-SHOCK PROTEIN HSP20 FAMILY // SUBFAMILY NOT NAMED; Heat shock protein 22D | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTTCAGTTTGCCTCAAGCTTACCCCACCACAAACACCATGGCCCTTA |
| Internal bar code: | TGTTATCCGTGAAGAATCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1518 |
| LEAP-Seq percent confirming: | 90.3226 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCTCACGAATTCGGCAA |
| Suggested primer 2: | GCCTCATCAGAGTGGCAAGT |