Insertion junction: CLIP2.071552_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.071552
Chromosome:chromosome 3
Location:5519784
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre03.g184950 MAW3 Membrane-associated hydroxyproline-rich glycoprotein 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCTACACCTTCCCAGACGGTGCGCTCGCGCCACCCGGCTACTACAAC
Internal bar code:GAACGTCTGGTTCGCGTAGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:403
LEAP-Seq percent confirming:61.5385
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGCCAGTCCTCAGTATC
Suggested primer 2:CAACAGGCCCACGAAACTTG