Insertion junction: CLIP2.071562_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071562
Chromosome:chromosome 4
Location:126342
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g216700 PHOX,PHO5 Phosphate-repressible alkaline phosphatase; (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGGTGCTGGGGGGCAGTGGGGTGTGTGCACTGGGCAGCACGCACTTC
Internal bar code:GCTTACTTTTTATACATTCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:241
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGATGGTGGTCTGGAAGG
Suggested primer 2:CCCTTACCCTCCTCTTCCCA