Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.071563 |
Chromosome: | chromosome 6 |
Location: | 8531979 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g309450 | CPZ | (1 of 1) 3.4.17.12 - Carboxypeptidase M | 3'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAAAACACACAGCCAGCAGAGCCTAACCGTCAAACCTCCATTTCCTTC |
Internal bar code: | GCGAGGAGCCCGTTGGTCACGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3368 |
LEAP-Seq percent confirming: | 68.254 |
LEAP-Seq n confirming: | 43 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTCGCGTGAAGTGTCTCAC |
Suggested primer 2: | GTTCTTCAACGGCACCACAC |