| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071564 |
| Chromosome: | chromosome 10 |
| Location: | 1278232 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426800 | (1 of 1) IPR000104//IPR003480 - Antifreeze protein, type I // Transferase | 3'UTR | |
| Cre10.g426850 | (1 of 3) PF02458 - Transferase family (Transferase) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTTTGAGCGCTCGGGTGTCGGGCGGAGCTATTGTGCGGCATAAGGTGC |
| Internal bar code: | ACTTCGGATCAGCACAGATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2383 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGTCAAGGCCTCTTCAA |
| Suggested primer 2: | TAAAGTTTGTGCAGCGTGCC |