Insertion junction: CLIP2.071578_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.071578
Chromosome:chromosome 9
Location:327542
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g800973 (1 of 2) PF00075//PF00078//PF03372 - RNase H (RNase_H) // Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCCAGCGTGGTTCACGACTATCTGACCAACTCGATTTTCAATCGCAG
Internal bar code:GGCTTGATGGCAGTAGAAGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2057
LEAP-Seq percent confirming:27.3973
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAACACTGCGAACCATTT
Suggested primer 2:TGTGGTTGCCAGGGTGATAC