| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071580 |
| Chromosome: | chromosome 16 |
| Location: | 5145196 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685400 | TIC32-3,SDR26 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.105//1.1.1.300 - All-trans-retinol dehydrogenase (NAD(+)) // NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGAGCAACACGATAGGATTGCCCCCGCTGAACATACGGTACACGGATT |
| Internal bar code: | CCGCGCGGTCTGGGCCCAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2212 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGGAGAGAGGAGTGGAA |
| Suggested primer 2: | GGCGTGAGTTTGGGATGGTA |