Insertion junction: CLIP2.071586_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.071586
Chromosome:chromosome 17
Location:2449348
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g715050 RAB28 Small Rab-related GTPase; (1 of 1) IPR001806//IPR003579//IPR020849//IPR027417 - Small GTPase superfamily // Small GTPase superfamily, Rab type // Small GTPase superfamily, Ras type // P-loop containing nucleoside triphosphate hydrolase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCTTGACACAGCCATAGCAGTAGAGGCCCACTCCATGGCCACGCACC
Internal bar code:AGGTACATGGGCTTATGTATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:520
LEAP-Seq percent confirming:35.7143
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGGCGTTGAAGAGGTAGG
Suggested primer 2:CCGACGTTGGTTGTGCAAAT