Insertion junction: CLIP2.071586_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071586
Chromosome:chromosome 17
Location:2449352
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g715050 RAB28 Small Rab-related GTPase; (1 of 1) IPR001806//IPR003579//IPR020849//IPR027417 - Small GTPase superfamily // Small GTPase superfamily, Rab type // Small GTPase superfamily, Ras type // P-loop containing nucleoside triphosphate hydrolase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATTGGTGTGTCCATTGCAGCCCCGAATAGCAAGTCACGCCGCGGCC
Internal bar code:AGGTACATGGGCTTATGTATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2287
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCGACGTTGGTTGTGCAAAT
Suggested primer 2:CTGGGCGTTGAAGAGGTAGG