Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.071589 |
Chromosome: | chromosome 14 |
Location: | 1788316 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g801621 | (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGCCCCACCTGAGCCGCCAGCGTCATTGCTGTTGAAAGTGACATTT |
Internal bar code: | GGGGGGGTCCAACTAGCGTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3002 |
LEAP-Seq percent confirming: | 95.8333 |
LEAP-Seq n confirming: | 23 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCTTGCCGTATGACACCTG |
Suggested primer 2: | TTATTGCCGCATTGCCGATG |