| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071612 |
| Chromosome: | chromosome 3 |
| Location: | 7988509 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201900 | RSP1 | (1 of 1) PTHR23084//PTHR23084:SF172 - PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED // SUBFAMILY NOT NAMED; Radial spoke protein 1 | 3'UTR |
| Cre03.g201950 | (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGCTAGATCGGGTAACAATTGCATGTATCATCAATACTTCGTGAGG |
| Internal bar code: | GATTAGGGAGAGAGTGATACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCGCCCAAGAAGAAG |
| Suggested primer 2: | TGGACAACCAGCTGTGTGTT |