Insertion junction: CLIP2.071621_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.071621
Chromosome:chromosome 9
Location:5070000
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g406050 DIV64,PYR7 (1 of 1) 6.3.4.2 - CTP synthase (glutamine hydrolyzing) / UTP--ammonia ligase; CTP synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGGCTGCCGTGCCATGTTGCTGTCAGTCCGGCCAGGTTTCCCCATCG
Internal bar code:TTAAAGGTGATCGGGTTCATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1711
LEAP-Seq percent confirming:81.3953
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGAGGCAGATCAGACGT
Suggested primer 2:TCCGGAGTGTGATGGCTAGA