| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071623 |
| Chromosome: | chromosome 1 |
| Location: | 7755878 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTCGCACGCCCCTGTTGCGCCTGATCGATGGACACGGCCCCCGCACA |
| Internal bar code: | ATAGGCGGCAGGACAGCTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 11.7647 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCTTCCACTCCAGCACG |
| Suggested primer 2: | GCCACAACGAACACAACCTC |