| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.071626 |
| Chromosome: | chromosome 1 |
| Location: | 6493882 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800117 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCCAGCGGCGAACACAGACGCTCCCATAAACCACTATTCTATTCACC |
| Internal bar code: | TTCGTGGTTGATTGCGTTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1617 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTTGTTAACCACACGCG |
| Suggested primer 2: | CCATTCTGCAACCGAGGTCT |