| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071640 |
| Chromosome: | chromosome 16 |
| Location: | 4177854 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670350 | (1 of 1) 2.7.1.81 - Hydroxylysine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACGACAATGCCCCACGCCCCCTCTGCTCCGTGTGAGGACGAGGTTCG |
| Internal bar code: | ACGGTCAGGTAGAATCAAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGATGAGGCCATGCTACG |
| Suggested primer 2: | CATGCACACACACACACAGG |