| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071642 |
| Chromosome: | chromosome 6 |
| Location: | 1717940 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262700 | QCR7 | (1 of 1) K00417 - ubiquinol-cytochrome c reductase subunit 7 (QCR7, UQCRB); Ubiquinol:cytochrome c oxidoreductase 14 kDa subunit, mitochondrial | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTTAATGGCGTCCCTCGCGTTCGATGTTCCGTGATACTGAGGTCCGA |
| Internal bar code: | GAGACGACCCGGGGGAGAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3133 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGTTGATTGAGGAGGCC |
| Suggested primer 2: | GAAGCTGTGCGGCCCTATAT |