| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071650 |
| Chromosome: | chromosome 12 |
| Location: | 2734453 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504400 | (1 of 1) 2.3.1.22 - 2-acylglycerol O-acyltransferase / Monoglyceride acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATACCAGTAACCCGCGCACCCTGCATGAGCGGCTGCTGCGGCTGCCG |
| Internal bar code: | GAAGATTGGCGTTTCACATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 59.2593 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGGCGCTTTATTACGG |
| Suggested primer 2: | ACATCGACCTCAGTCATGGC |