| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.071659 |
| Chromosome: | chromosome 2 |
| Location: | 2584458 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092900 | GUA1 | (1 of 1) 6.3.5.2 - GMP synthase (glutamine-hydrolyzing) / GMP synthetase (glutamine-hydrolyzing); GMP synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTAAAACCCCAAGGCTCTCAATTGAGTCGCCTTGAATGGCCAATTC |
| Internal bar code: | CCGTGTACATGGATTTTCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTCTCTTGCCTTGTGCC |
| Suggested primer 2: | AGTCAAACAGCTGGGACGAG |