| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071687 |
| Chromosome: | chromosome 6 |
| Location: | 3184554 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCGTGTCACTCCTTGGGAAAGGCAGTCCCCGACCAGGTCGCAAAA |
| Internal bar code: | TCCCCGACAGATCAGTGTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4021 |
| LEAP-Seq percent confirming: | 22.9167 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGACCTCGAAGTGAACC |
| Suggested primer 2: | CATCCTACGCCATTTGCACG |