| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071707 |
| Chromosome: | chromosome 14 |
| Location: | 2054226 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621250 | (1 of 2) K09668 - glycosyltransferase-like protein LARGE (LARGE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGTCACCTGCTCGTCCCGAACAGCCAAACCGTAATCCCAACTATAAT |
| Internal bar code: | AGTAAATTTCGTAATGAGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1433 |
| LEAP-Seq percent confirming: | 91.8367 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTGCCTTTCCCCTGAAT |
| Suggested primer 2: | TCATCGAGCATAGCACGCAT |