Insertion junction: CLIP2.071729_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.071729
Chromosome:chromosome 6
Location:5730036
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g288400 uS11m,MRPS11 (1 of 3) PF00411 - Ribosomal protein S11 (Ribosomal_S11); Mitochondrial ribosomal protein S11 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATGTGATTCGCCGCATCAACTCATCGCTTCGCCCCATCCACGCATCAC
Internal bar code:GGGTGGAGTAGTGAAACCCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1892
LEAP-Seq percent confirming:98.1481
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCTAGGCTACTCGTCGG
Suggested primer 2:TTGATAGCTCGATTCGGCCC