| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071734 |
| Chromosome: | chromosome 12 |
| Location: | 9379201 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGGCTGCAACAGGCTGTCAAGATGGGTTCAGTACGGATTGCTTTGAC |
| Internal bar code: | TTGCCTCGTAGGTATAAGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 191 |
| LEAP-Seq percent confirming: | 40.7407 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAACACCCGTGCAACAC |
| Suggested primer 2: | CGGTGACATGCATCATGCTG |