| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071735 |
| Chromosome: | chromosome 10 |
| Location: | 3171721 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441800 | (1 of 4) PF05066 - HB1, ASXL, restriction endonuclease HTH domain (HARE-HTH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGGCTGTTTCTGAAACCACGCTCCCGAAGCTCTTGACGCCACGGGTC |
| Internal bar code: | GGGGCGTATATCGCCAGGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5275 |
| LEAP-Seq percent confirming: | 95.1613 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATGGGAAAAGTGGGCTGC |
| Suggested primer 2: | GCGCTGCAAATTCAGACACA |