| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071753 |
| Chromosome: | chromosome 16 |
| Location: | 791638 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690700 | (1 of 13) IPR000086//IPR015797 - NUDIX hydrolase domain // NUDIX hydrolase domain-like | 3'UTR | |
| Cre16.g690750 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGAGGAGGAGGAGGAGCATGAGGGGGTGGAGGAAGAGGACGAGGAG |
| Internal bar code: | TAATCACCGGTGGTATCTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 3.7037 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATCCACACTACACCAC |
| Suggested primer 2: | ACACACACACACACACACCT |