| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071762 |
| Chromosome: | chromosome 6 |
| Location: | 3745085 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278163 | ARG9 | (1 of 1) 2.6.1.11 - Acetylornithine transaminase / Succinylornithine aminotransferase; Acetylornithine aminotransferase | 3'UTR |
| Cre06.g278164 | (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCATGCGCGCATCTATCGTTTCATCGCAACGGGATTGTGTACTTTGG |
| Internal bar code: | TTACCCCGTGTAGTTCATCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1845 |
| LEAP-Seq percent confirming: | 98.4848 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCGGTGTTGAGTTGGAT |
| Suggested primer 2: | TCATTCAACCCCGCATCACA |