| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071776 |
| Chromosome: | chromosome 2 |
| Location: | 8083136 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144350 | RJL1 | Small Rab-related GTPase; (1 of 1) K19372 - DnaJ homolog subfamily C member 27 (DNAJC27) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGCCACACTTCCTCGGCATTCGGTGGGCTAGTTGCTGGTTATGGGC |
| Internal bar code: | TAGACGAAGCCGGTAATTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 92 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAATGCTGCCGGGAAATC |
| Suggested primer 2: | CTATGCTCCGAGCTCTGTGG |