Insertion junction: CLIP2.071778_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071778
Chromosome:chromosome 9
Location:1390543
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g400150 (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTAGCCCCGCGGGCTTCTCTGAGTGCATTTGTGATCCGCTGGCTATTC
Internal bar code:GTGTGTTTATGTGTGAGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:752
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGGTGGGCTGGATATCG
Suggested primer 2:CTCTGAAGGAGGCGTCCAAG