Insertion junction: CLIP2.071799_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.071799
Chromosome:chromosome 13
Location:3117994
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g584700 (1 of 2) PF02636 - Putative S-adenosyl-L-methionine-dependent methyltransferase (Methyltransf_28) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCCAGGATGAAGTCCAGCACCCTGTTGAAGCGCTCGTCCAGCTGCTGC
Internal bar code:CAGGCAAGTTGGGCGCAATAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:550
LEAP-Seq percent confirming:30.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGGAGGTGCTGGACAAC
Suggested primer 2:GTGAAGTCGTAGGTGGGGTG