| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071801 |
| Chromosome: | chromosome 16 |
| Location: | 6072788 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677700 | NUP62 | Nucleoporin 62; (1 of 1) K14306 - nuclear pore complex protein Nup62 (NUP62, NSP1) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCACCTGCGCTGTGTGTGTGTGTGTGGGGGGGGGGGGCGGGAGGGA |
| Internal bar code: | TATAGGATTATCCCCTGTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2259 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACATGCGCTTTGACAC |
| Suggested primer 2: | TTCAAATGCGGGCTGGTTTG |