Insertion junction: CLIP2.071802_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.071802
Chromosome:chromosome 10
Location:5000824
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g454400 (1 of 1) K10632 - BRCA1-associated protein (BRAP) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCTCCCGTCTGGAGGTCCAGGCCCGGGCACGGGCCTGGCTCCTGGCT
Internal bar code:GTTCTATGAACTCCTAGCTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1898
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAAGTCGGCGATGGTCAT
Suggested primer 2:TGCAACATTCGAGCACTCCT