| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071802 |
| Chromosome: | chromosome 10 |
| Location: | 5000830 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454400 | (1 of 1) K10632 - BRCA1-associated protein (BRAP) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAATCTGGACCGCTGAGCCCGAGCGAGGAGTGTAGCAGCGAGATCTAA |
| Internal bar code: | AGCGATGGATGTGTATAGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2829 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACATTCGAGCACTCCT |
| Suggested primer 2: | CAGAAGTCGGCGATGGTCAT |