| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071803 |
| Chromosome: | chromosome 7 |
| Location: | 459471 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315400 | NOA2,NOL1,YQEH | putative ribosomal 30S subunit assembly factor; (1 of 1) PTHR11089:SF3 - NITRIC OXIDE-ASSOCIATED PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGTTGGGTGTGTGCGGCCTGCCCTTGATGAGGATCAGTTCCTGCATT |
| Internal bar code: | TGGTCTGACTAGCTGTTTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3040 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCATCCATCACAACCTG |
| Suggested primer 2: | CTGACCGTGTTCGTCAGTCA |