| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071810 |
| Chromosome: | plastome |
| Location: | 76887 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802295 | ChreCp030,psbN,2717031 | photosystem II protein N; (1 of 1) K02715 - PsbN protein (psbN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCTCGACAAAATGACTTTCATAAAAATATAAAGTAGTTAGCTACTTA |
| Internal bar code: | GGCCATAAGGGATGCGGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 60.8696 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCAGTCCCTTTTCCCC |
| Suggested primer 2: | GAAGGGGAAGGACGTCAGTG |