| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071812 |
| Chromosome: | chromosome 16 |
| Location: | 5593642 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681400 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGCGCCAACTGCAACAAGGCAACGTTTTAGAGTTGTCCGTTGCCAG |
| Internal bar code: | GATGCCCGGGGAGAACGGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5431 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAACTCCTGCACGGCAAT |
| Suggested primer 2: | AGTTTGGGCTTACGGAGTGG |