| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071818 |
| Chromosome: | chromosome 12 |
| Location: | 4549734 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521350 | SLY1 | (1 of 1) PTHR11679:SF2 - SEC1 FAMILY DOMAIN-CONTAINING PROTEIN 1; SM/Sec1-family protein, SLY1 homolog | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTGGCATTGTCTTCTCGTCTCACATACGAGAAATATAAGCGTAACCC |
| Internal bar code: | GGAAGCAATTTCTAGTTTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5047 |
| LEAP-Seq percent confirming: | 68.0556 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCTCCACCTGATACCCC |
| Suggested primer 2: | CTGACAACTCACCAGGACCC |