| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071822 |
| Chromosome: | chromosome 12 |
| Location: | 231078 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484900 | FAP83 | (1 of 2) PF13879 - KIAA1430 homologue (KIAA1430); Flagellar Associated Protein 83 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTTTTAGCCGGGGGCTGCATGGAGCGACTTGATGAGTTGCGGGCTTC |
| Internal bar code: | CAAACGAGAACTGAATATAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1317 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAAGTGCCGACTGCGAGT |
| Suggested primer 2: | CTCCAGACCTACCCTGTCGA |