Insertion junction: CLIP2.071823_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.071823
Chromosome:chromosome 16
Location:7978850
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g691552 NDA1 Type-II NADH dehydrogenase; (1 of 3) K17871 - NADH:ubiquinone reductase (non-electrogenic) (ndh1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAGGCTCCGCAGCGGCATCAGGTTGTCACGCACGCGGCTTCAATGGCG
Internal bar code:ACTTGACGGAACTGTCTGACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1404
LEAP-Seq percent confirming:16.9231
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCGTGTGCTCAAATATG
Suggested primer 2:CCTCCCACGCTTTGTTTGTG