Insertion junction: CLIP2.071830_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.071830
Chromosome:chromosome 1
Location:5663006
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040500 (1 of 1) K10643 - CCR4-NOT transcription complex subunit 4 (CNOT4, NOT4, MOT2) outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAAGACGCTGCTGCTGTTGCTGGTATTGGTTGGCGATAAATTACATTG
Internal bar code:ATGCTGCTTTCACTTTCGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1327
LEAP-Seq percent confirming:98.6301
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:AGAACAAGAGCCAGAGTCGC
Suggested primer 2:CAAACATCTAGAAGCGCGCC