Insertion junction: CLIP2.071836_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.071836
Chromosome:chromosome 14
Location:3108823
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g628350 CLPQ,HSLV1 ATP-dependent subunit of mitochondrial HslUV protease; (1 of 1) K01419 - ATP-dependent HslUV protease, peptidase subunit HslV [EC:3.4.25.2] (hslV, clpQ) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGATGGAACCCACGAGGTTCTTAACGAGGTTCTCCAAAGCTAAGTACAG
Internal bar code:AGTTTTACATTTCAAAGTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1515
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTGACCAGCTTCCTCAA
Suggested primer 2:GAATGACGGGGCCCAGTAAA