Insertion junction: CLIP2.071841_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.071841
Chromosome:chromosome 13
Location:2699845
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g581650 PRPL7,L12 (1 of 1) PF00542//PF16320 - Ribosomal protein L7/L12 C-terminal domain (Ribosomal_L12) // Ribosomal protein L7/L12 dimerisation domain (Ribosomal_L12_N); Chloroplast ribosomal protein L7/L12 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCGCAGTGACGCACACGCCCCTAACCACCCCACAGCGGCGACCCGGC
Internal bar code:GATCTGTATCGTCAGTGTGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:437
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TCTACAAGGTGGTCCGCAAC
Suggested primer 2:TACCGTAATCGCATCAGGGC