| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071887 |
| Chromosome: | chromosome 15 |
| Location: | 1745036 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g751197 | (1 of 1) K14794 - ribosomal RNA-processing protein 12 (RRP12) | outside_mRNA | |
| Cre19.g751247 | (1 of 1) K16742 - ADP-ribosylation factor-like protein 2-binding protein (ARL2BP, BART) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGGTCACAAGTTTTAACACTACGTAGCGCTGCTGAAAAAGAGGGCCT |
| Internal bar code: | TTCATGCTCGTTCGAGGACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1238 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACCCACTTTGCAAGGC |
| Suggested primer 2: | GTATCCTACACCGACAGGCG |