Insertion junction: CLIP2.071900_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.071900
Chromosome:chromosome 7
Location:1723648
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g325716 DIV146,DNL1,LIG1 DNA ligase 1; (1 of 1) K10747 - DNA ligase 1 (LIG1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTCCTGCCGCTGTCCCTGGCCCATCATCACCACAGCTGTCCTTTCCGC
Internal bar code:CTCTCTATCAGGAACGGTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTAGAGACAGTCGAAGGCG
Suggested primer 2:ATCTACAACCCCTCTCCCCC