| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071907 |
| Chromosome: | chromosome 14 |
| Location: | 703578 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612500 | (1 of 1) K05954 - protein farnesyltransferase subunit beta (FNTB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCAATCCCCAAAGAGCGCAGCTGTCAACTGGAATCATCTCCCCCGGC |
| Internal bar code: | GTAAAATCGCGTAGGTAGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGTGGGAGGCATTGGG |
| Suggested primer 2: | CAGCCACCAACTGCCTTTTC |