| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071915 |
| Chromosome: | chromosome 6 |
| Location: | 5340444 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTAGCCGCCCGAGGCTGAAAAGGAACTGCCCACTTCGTGCAGCGGAC |
| Internal bar code: | GATCGAAAAATGTGTTCGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2949 |
| LEAP-Seq percent confirming: | 23.8806 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCAAAGAATCCTCCATG |
| Suggested primer 2: | AGGCGCTCAAGATCATCGAG |