| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071918 |
| Chromosome: | chromosome 6 |
| Location: | 8258513 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306750 | FAP290 | Flagellar Associated Protein 290; (1 of 1) K13448 - calcium-binding protein CML (CML) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACGCATTCCAATTTGGGACGTGCTGTGCCCAGCGTCCACTAGTACA |
| Internal bar code: | GGAGTGACTCGTGGTGGTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1430 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCTGACACCTACATTGC |
| Suggested primer 2: | GCCTTTGATTGTGTGCCGTT |